FastQCFastQC Report
Wed 20 Jul 2022
HGCNNBGXM_n01_12275_totalRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCNNBGXM_n01_12275_totalRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18912060
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT5477882.896500962877656TruSeq Adapter, Index 16 (97% over 40bp)
CCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCAT1731140.9153630011749117No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC1663190.8794335466363792No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACG1648800.8718246452263793No Hit
TCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC1620390.8568024847636904No Hit
CCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCA1568240.8292274876454495No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC1114370.5892377668006553No Hit
CTTCCTGTGGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAAG986040.52138159460154No Hit
CCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC954410.5046568168671207No Hit
TTCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGA902700.4773144755251411No Hit
ATTCCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTG860120.4547997415405831No Hit
CCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGA842870.445678577584885No Hit
GCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGG811880.42929220825230036No Hit
GCCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGAT798250.4220851668194792No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT762000.4029175034343165No Hit
GCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAAGGTATT742560.3926383482285907No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTT740090.3913323033027602No Hit
CCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCA734890.3885827350378541No Hit
GCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCCC733590.3878953429716276No Hit
CCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATT732650.38739830563143307No Hit
TCCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTC656680.34722817080741075No Hit
GCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC629530.3328722518858337No Hit
GCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGT625300.33063558385495817No Hit
CTCCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGT624210.33005923204558363No Hit
CTCTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGCTA613940.32462883472239407No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACT612210.32371407451118495No Hit
ACCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC611990.3235977466230543No Hit
TCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAG609380.32221767485932257No Hit
CCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGT585370.3095220721592465No Hit
GCCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAA577500.3053607063429367No Hit
CCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAA570150.30147429735311754No Hit
CCCCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATA569480.3011200260574469No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG558910.2955309997958974No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGC548600.2900794519475932No Hit
TCCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACC546300.28886329675350014No Hit
CCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGG530810.2806727559028472No Hit
TCCTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGACGGAG524620.27739971214135317No Hit
CCTTCCTGTGGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAA519640.27476647176457775No Hit
CCTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGACGGAGT518300.27405792917323657No Hit
CACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTT507200.26818865845391776No Hit
CCCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGTGGA503850.2664173019755648No Hit
GCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC502610.2657616356970103No Hit
CGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCT497790.2632129974206935No Hit
GCCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGA495560.26203385564555104No Hit
CCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCTT493160.2607648241386713No Hit
CCCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAG478960.2532563877229662No Hit
CCCCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGCTTTC475880.2516277972891372No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTG471200.24915318585072171No Hit
ACCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGA470110.24857683404134714No Hit
TCCCCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGAT464830.24578496472621175No Hit
TCCCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGTGG418970.22153588768225146No Hit
CCACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACT401760.212435874251668No Hit
GCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTC394870.20879269630066738No Hit
CCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAG394190.20843313737371813No Hit
CCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTC388730.20554609069556676No Hit
GCGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCC387780.20504376572409352No Hit
CCCCAGCACGACGGAGTTTCACAAGATTACCAAGACCTCTCGGCCAAGGT382300.20214614378338477No Hit
CCCTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGACGGAG380240.20105689173997968No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG377060.19937542499336403No Hit
TTCCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGC369930.19560534389167547No Hit
TTCCTGTGGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAAGG368110.19464299499895832No Hit
CCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGAG363690.19230586197378816No Hit
TGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT360340.19053450549543519No Hit
CTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCT346830.183390915637958No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA338060.1787536630065683No Hit
TTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTT336610.17798695647116178No Hit
CCCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACC328420.1736563864539347No Hit
CCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCCCG321840.17017712507257274No Hit
CGCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCC320130.1692729401239209No Hit
GCTACTACCACCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGG320060.16923592670497029No Hit
CGCCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACG307370.16252592261234367No Hit
ATCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTATTC304960.16125160347418527No Hit
TGCCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGA302520.15996142144219086No Hit
CTCCTCCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTG300140.15870296519786845No Hit
GCGCCCGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC299950.1586025002035738No Hit
CCTCCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTGAA299590.15841214547754184No Hit
GCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGA297490.15730174290902207No Hit
CCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAA296690.15687873240672884No Hit
TGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC296120.1565773374238449No Hit
AGCGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGC291200.15397582283474143No Hit
CGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAAGGTATTT290980.15385949494661078No Hit
GCCGCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGC290440.15357396285756286No Hit
CCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAA289440.1530451997296963No Hit
GTCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCA285820.15113107720681934No Hit
CCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTA282890.14958180124217033No Hit
CCCTTTTGTTCTACTGGAGATTTCTGTTCTCCATGAGCCCCCCTTAGGAC278310.1471600661165415No Hit
CCCCCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGAT277300.14662601535739628No Hit
TCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTTT277240.14659428956972428No Hit
AGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC276800.146361633793463No Hit
CCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGA275570.14571125514618713No Hit
ACCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAA272740.14421485549432478No Hit
TCCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCT271820.14372839341668756No Hit
TGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT270650.14310974055708367No Hit
ACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTT263780.13947713786864044No Hit
GTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGG263600.13938196050562446No Hit
GCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCC258270.1365636530340957No Hit
ACCCTTTTGTTCTACTGGAGATTTCTGTTCTCCATGAGCCCCCCTTAGGA255220.1349509254941027No Hit
GGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGC254620.13463366761738277No Hit
GGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAA253480.1340308776516149No Hit
CCACCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGGTCTAGGC253040.13379822187535362No Hit
TGCGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGC252590.13356027846781365No Hit
GTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCT245360.12973732105333843No Hit
CCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTTTT244660.12936718686383186No Hit
CTCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGA244470.12926672186953722No Hit
TGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAG243560.12878554742317866No Hit
CCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTC243260.12862691848481866No Hit
GTTCCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTG236950.12529042314798072No Hit
GCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCA236740.12517938289112873No Hit
GTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCAC235940.12475637238883548No Hit
CCTCATAAAACTGATACGAGCTTCTGCTATCCTGAGGGAAACTTCGGCAG235470.1245078537187382No Hit
CCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC235150.12433864951782089No Hit
CTCCGGAATCGAACCCTTATTCCCCGTTACCCGTTGAAACCATGGTAGGC234250.12386276270274099No Hit
CACTCTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGC232040.12269419619015591No Hit
CTCCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTGAAG227670.12038350132137907No Hit
ATTCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCG227610.12035177553370706No Hit
TGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCC221870.11731667517975303No Hit
ACCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAG219380.1160000549913653No Hit
CCGCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCC218560.11556646922651471No Hit
CCCTCACTACCCGACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATC217560.11503770609864816No Hit
ACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGAC217300.11490022768540285No Hit
ATCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGA216840.11465699664658426No Hit
TCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTT216640.11455124402101093No Hit
TCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGA215820.11411765825616035No Hit
CCGTCAGTGTAGCGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGAC214610.11347785487144182No Hit
ACCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACC214510.11342497855865517No Hit
GTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCG213270.11276931228010062No Hit
GCGCTTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC212640.1124361915095447No Hit
CGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT211380.11176994996843284No Hit
ACCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTC209610.11083403923210904No Hit
CCCCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGTGG208690.11034757715447181No Hit
TCCTCCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTGA207720.10983467692044124No Hit
CTTCCCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGT204270.10801044412930161No Hit
CCCCCGGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCAT200980.10627081343862065No Hit
CCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTT198250.10482729009954495No Hit
TTTCCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTG195600.10342606781069856No Hit
GCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCC193370.10224692603555616No Hit
GCCTCGAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGAT191280.10114181109831505No Hit
TTCCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCAC190030.10048085718848185No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)