FastQCFastQC Report
Wed 20 Jul 2022
HGCNNBGXM_n01_12271_totalRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCNNBGXM_n01_12271_totalRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17787776
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC5817533.2705212838299738TruSeq Adapter, Index 12 (100% over 50bp)
ATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAG3134271.7620359060064619No Hit
CAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCAC2127121.1958324638223463No Hit
CCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTA1850631.0403942572697116No Hit
CCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTAA1567070.880981411054423No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT1426990.8022307004540646No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGC1406900.7909364273532565No Hit
CTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTC1170860.658238556635748No Hit
ACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCA1117510.6282460494218052No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA1101760.6193916541337152No Hit
CTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGATAA1089630.6125723643023164No Hit
AAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACC991000.5571241733648996No Hit
CTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGCTATC983870.553115802672577No Hit
GTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACT980010.5509457730972102No Hit
CCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAA971510.5461672105607807No Hit
CGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTA960260.5398426424978592No Hit
CCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCA944550.5310107345628818No Hit
CCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCTT943720.5305441219857953No Hit
CGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTA921900.5182772708628668No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC910390.5118065350047133No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACT879530.49445754207833514No Hit
CTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCT859460.48317451265408334No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTG851320.47859833629566734No Hit
CCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAATA848360.47693427216533424No Hit
CTTTTGTTCTACTGGAGATTTCTGTTCTCCATGAGCCCCCCTTAGGACAT832440.4679843056265156No Hit
CCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTA792290.4454126249397339No Hit
CCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAAT764670.42988510761547705No Hit
GCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTT754730.42429700036699364No Hit
CGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCT737350.4145262454395648No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC706150.39698611001172934No Hit
CCCTTTTGTTCTACTGGAGATTTCTGTTCTCCATGAGCCCCCCTTAGGAC700770.39396156101808344No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTT645480.36287841717817904No Hit
GTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTAC641580.36068590024969954No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG637630.35846527412982937No Hit
CCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTATTCTA628920.3535686529895587No Hit
CCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTT617690.3472553286031936No Hit
GTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCAC609010.34237557297775734No Hit
ACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAG592060.332846557096289No Hit
CGCCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACG589080.33117124928939967No Hit
TACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGC565170.3177294339663373No Hit
GGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGC555330.3121975451006354No Hit
CTCGTTCGTTATCGCAATTAAGCAGACAAATCACTCCACCAACTAAGAAC553480.3111575050191772No Hit
TTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTT544300.30599665747983334No Hit
GCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGT538390.3026741510574453No Hit
CGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAAGGTAT538070.30249425223254445No Hit
CCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAA517770.2910819205278951No Hit
GCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAG512680.28822040484431555No Hit
ACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCC510680.2870960371886851No Hit
GCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAA477190.2682685007951528No Hit
CCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGTGGAA471710.2651877334187253No Hit
CCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGATA470600.2645637093698504No Hit
CCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTC467340.26273099009117273No Hit
CCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGG466030.2619945292767348No Hit
CGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACAGTTATACCATGT463940.2608195650766009No Hit
GCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGG455540.25609722092295295No Hit
CCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATT435010.24455558693790613No Hit
CTCTTACTCAAATCCATCCGAAGACATCAGGATCGGTCGATTGTGCACCT423490.23807922924147462No Hit
ATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCA422420.23747769254571227No Hit
CAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTAAA417780.23486915958464957No Hit
CAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCT416850.2343463286247814No Hit
GCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCCC411060.2310912842617312No Hit
CTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTTTTCA408180.2294721948376233No Hit
CCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGT404420.22735838364503802No Hit
CCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGAGCC380020.21364109824634625No Hit
GCCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGA379580.21339373736210754No Hit
CCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTGAAGAA368350.20708041297574242No Hit
GTCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCA363430.20431446854289148No Hit
CTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCA361310.20312263882792317No Hit
GCGGTAACATTCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA350290.19692737304539926No Hit
CCCGACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTATTTTGCC349440.19644951679175632No Hit
GCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTC343870.19331815287082543No Hit
CAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAAG343260.19297522073585816No Hit
CTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACTA341030.19172155079983016No Hit
GCCGACTTCCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCTT331240.18621777112551902No Hit
CACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTT323410.1818158717537257No Hit
CCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCC318980.17932539739650422No Hit
CGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCA315710.1774870562795484No Hit
GCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCC311200.17495160721610167No Hit
CCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCAT309970.17426012110788894No Hit
CCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTT304750.1713255215266934No Hit
TGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAA304230.17103318593622946No Hit
GCGGCATATAACCATTATGCCAGCATCCTTGACTTACGTCGCAGTCCTCA303240.17047662394669238No Hit
GTACTTTTTCACTCTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTA300410.16888564371397527No Hit
CGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGACGGAGTTTC298650.16789620017702045No Hit
CTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGCTTTCGCA295740.1662602452380781No Hit
GCGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCC293870.16520896148006362No Hit
CTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC290430.1632750491123792No Hit
CCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTC286490.16106004483078717No Hit
GGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAA286280.16094198622694594No Hit
CACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGC282130.15860892334151272No Hit
CCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCTTGGAGACCT279860.15733276605237215No Hit
CTCTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGCTA278770.15671998568005352No Hit
CCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTAAAAGCTCTTCA276190.15526955140429022No Hit
GCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC272170.15300957241647298No Hit
CCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAA271670.15272848050256535No Hit
CTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGACGGAGTT271000.15235181733792916No Hit
CCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAAT270300.15195828865845848No Hit
CGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCGCTGC264880.1489112523116999No Hit
CTTCCTGTGGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAAG263260.14800051451063923No Hit
GTGGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAAGGTGCTG258010.14504904941460922No Hit
CTCCACATTCAGTTACGTTACCGTGAAGAATCCATATCCAGGTTCCGGAA256000.1439190599207006No Hit
CTCAATCTGTCAATCCTTATTGTGTCTGGACCTGGTGAGTTTCCCCGTGT255690.14374478293407786No Hit
CGCGTCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT254050.14282280145646087No Hit
GGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTC250870.14103505688400844No Hit
CTCGAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGATAA250390.14076520864665712No Hit
CTAGAAACCAACAAAATAGAACCAAACGTCCTATTCTATTATTCCATGCT248640.13978138694798045No Hit
CCCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAG247460.13911801003115848No Hit
CCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGCCATAATCCAGC237700.13363109587168176No Hit
GCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTT235140.13219190527247476No Hit
GCCGCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGC233300.13115748702929472No Hit
GTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACAGTTATACCAT231830.13033107680240633No Hit
CCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTTTTC228800.12862765980412616No Hit
CCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCCCG227730.12802612310836387No Hit
CCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGT227360.12781811509207222No Hit
CCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAA226610.12739647722121078No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAAATTCGACGATCGATTTGCAC223740.12578300963538108No Hit
CTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATC223680.12574927860571217No Hit
CTCCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGT222950.12533888441140703No Hit
CCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGT221840.12471486036253211No Hit
CAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGC220300.12384909726769663No Hit
CGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCCA220270.12383223175286219No Hit
GCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCA219930.12364108925140502No Hit
GCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAAGGTATT218670.12293273762835782No Hit
CCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGA217570.12231433541776106No Hit
GCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGA216850.12190956306173407No Hit
CCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGCTTTCGC215180.12097071606928264No Hit
TCTCAATCTGTCAATCCTTATTGTGTCTGGACCTGGTGAGTTTCCCCGTG214530.12060529658120274No Hit
CTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGAGCCT213290.11990818863471184No Hit
CTGATGAGCGTGTATTCCGGCACCTTAACTCTACGTTCGGTTCATCCCGC212230.1193122737772277No Hit
GTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCAT210990.1186151658307368No Hit
AGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAAGT210400.11828347737232581No Hit
CCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCA209400.11772129354451057No Hit
CTCCTCCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTG209290.11765945332345089No Hit
CTCTCAATCTGTCAATCCTTATTGTGTCTGGACCTGGTGAGTTTCCCCGT208570.11725468096742392No Hit
CTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTTGAGCT208340.11712537868702642No Hit
GGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTT205420.11548380190980594No Hit
CCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTAC205280.11540509617391179No Hit
CCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGAG203280.11428072851828132No Hit
GCCCAGTGAAATGCGAGATTCCCCTACCCACAAGGAGCAGAGGGCACAAA201730.11340934358516772No Hit
GCTACTACCACCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGG199370.11208258975152374No Hit
ACCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGGTCTAGGCTT196720.11059280260781337No Hit
CCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTTG196630.11054220606330999No Hit
CTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCC196270.11033981988529651No Hit
CCGACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTATTTTGCCG196210.1103060888556276No Hit
GTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTA195900.11013181186900486No Hit
CTCCAATTGTTCCTCGTTAAGGTATTTACATTGTACTCATTCCAATTACA195880.11012056819244857No Hit
CCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGGTCTAGGCTTC195110.10968768664503083No Hit
CTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTAC192820.10840028567933394No Hit
GCCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAA192110.10800113516158513No Hit
GCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC192020.10795053861708175No Hit
GGCCCAGTGAAATGCGAGATTCCCCTACCCACAAGGAGCAGAGGGCACAA191060.10741084214237913No Hit
CGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAG190490.10709039736052443No Hit
GCCCTTTTGTTCTACTGGAGATTTCTGTTCTCCATGAGCCCCCCTTAGGA190110.10687676750595465No Hit
CTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGTTCGCCAAG187830.10559498837853591No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCAGA184920.10395903343959358No Hit
CTTAGGACATCTGCGTTATCGTTTTACAGATGTGCCGCCCCAGCCAAACT181650.10212069232263775No Hit
CCCCGCTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCA181530.10205323026329992No Hit
CGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAAATTCTCCGCTCTG181250.10189581879151166No Hit
CTCCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTGAAG179380.10084453503349718No Hit
GCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT179180.10073209826793413No Hit
CTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTT178170.10016429260184072No Hit
ACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTT177890.10000688113005246No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)