FastQCFastQC Report
Wed 20 Jul 2022
HGCNNBGXM_n01_12270_totalRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCNNBGXM_n01_12270_totalRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30632545
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC25006008.163213340582704TruSeq Adapter, Index 7 (100% over 50bp)
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGC6208702.0268312672029047No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT3651981.1921895487299539No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC3462271.1302586840238054No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTG3032800.9900581228232914No Hit
GTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACT2855650.9322274724480124No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACT2615880.8539545114517908No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTT2430670.793492672580747No Hit
ACGCAAAATGACATTGCAATCCGCCAGCAAGCACCCAAGGCCTTTCCGCC2160120.705171574872411No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG1948790.6361828571540498No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA1883160.6147579314745151No Hit
GGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGC1881110.6140887085940786No Hit
ATACAAATGATTTATCCCCACGCAAAATGACATTGCAATCCGCCAGCAAG1762900.5754990321568123No Hit
CCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTA1752130.571983163658129No Hit
GCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTT1732800.5656728815708914No Hit
CCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAAT1720120.5615334932177526No Hit
ACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAG1707970.5575671234629705No Hit
CCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTAA1673850.5464286431310229No Hit
GCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCC1436330.46889019505235363No Hit
CGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCT1431600.46734608567456604No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC1413890.46156465288796605No Hit
GCGGCATATAACCATTATGCCAGCATCCTTGACTTACGTCGCAGTCCTCA1379430.45031517949292166No Hit
GCCGCTTCACTCGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCTC1366200.44599624353771455No Hit
CAAATGATTTATCCCCACGCAAAATGACATTGCAATCCGCCAGCAAGCAC1342730.4383344576821808No Hit
CCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAA1336150.4361864154610725No Hit
GCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTC1228380.4010048789612486No Hit
GCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC1226450.40037483010308156No Hit
CCCCACGCAAAATGACATTGCAATCCGCCAGCAAGCACCCAAGGCCTTTC1222740.39916369991458434No Hit
GCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCTCCGCTTATTGATA1142310.3729073114884839No Hit
GTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTAC1127800.36817051929573597No Hit
CAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCT1109390.3621605713792308No Hit
CTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTC1088450.3553247044932114No Hit
CCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGCCATAATCCAGC1086920.3548252357092759No Hit
CCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCA1078280.3520047061058753No Hit
CGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCGCTGC1071510.349794638349507No Hit
CGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTA1034820.337817181040622No Hit
CGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAAGGTAT993820.32443272343189244No Hit
ACAAATGATTTATCCCCACGCAAAATGACATTGCAATCCGCCAGCAAGCA983710.3211323120556911No Hit
CCCACGCAAAATGACATTGCAATCCGCCAGCAAGCACCCAAGGCCTTTCC964660.31491343602041555No Hit
CGCCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACG935380.30535497458666916No Hit
GCGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCC928550.3031253198191662No Hit
CGCAAAATGACATTGCAATCCGCCAGCAAGCACCCAAGGCCTTTCCGCCA894650.2920586585280459No Hit
CTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGATAA820490.26784911276552437No Hit
GCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTT816640.26659227955104614No Hit
GCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAG804800.2627271093537935No Hit
CACGCAAAATGACATTGCAATCCGCCAGCAAGCACCCAAGGCCTTTCCGC790370.25801643317589185No Hit
CCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCTT774320.2527769076973526No Hit
GCCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGA764030.24941773528774705No Hit
GCGGTAACATTCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA762600.24895091152236942No Hit
CCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAATA745280.24329679430814513No Hit
GCTACTACCACCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGG742670.24244475932378456No Hit
CTCTTACTCAAATCCATCCGAAGACATCAGGATCGGTCGATTGTGCACCT736070.24029018809896469No Hit
CCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTT735880.2402281625637047No Hit
CGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTA726270.23709097628029271No Hit
GCGCCATCCATTTTCAGGGCTAGTTCATTCGGCCGGTGAGTTGTTACACA712040.23244559013950683No Hit
GCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGT682480.2227957226537984No Hit
AAATGATTTATCCCCACGCAAAATGACATTGCAATCCGCCAGCAAGCACC660380.21558117355250764No Hit
CCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTA659770.21538203893930458No Hit
CGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCA647570.2113993466752436No Hit
TGCCGCTTCACTCGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCT647280.21130467612142578No Hit
CCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAA639450.208748571168344No Hit
TGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAA638970.2085918750792662No Hit
GTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCG634410.20710326223302697No Hit
GTCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATCCGCCA623030.2033882591211406No Hit
GTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTA619340.20218365793635493No Hit
CTCGTTCGTTATCGCAATTAAGCAGACAAATCACTCCACCAACTAAGAAC592010.1932617743644872No Hit
GCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCA588850.19223019177805825No Hit
CTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCT583830.19059141184645284No Hit
CTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGCTATC582060.19001359501797843No Hit
GCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGG572380.18685355722157593No Hit
GTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGT550880.1798348782316324No Hit
CCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGTGGAA531750.17358988618151056No Hit
CCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCAT529710.17292392780292987No Hit
CGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTT524780.17131452838802652No Hit
GCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAAGGTATT517730.1690130545796962No Hit
CGCTTCACTCGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCTCCG505020.16486387272099004No Hit
CGCGTCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT501030.16356133648053076No Hit
TACAAATGATTTATCCCCACGCAAAATGACATTGCAATCCGCCAGCAAGC495700.1618213569913959No Hit
GTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCT495470.1617462734487128No Hit
GCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT487550.15916078797892894No Hit
CGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACAGTTATACCATGT481370.15714332583205215No Hit
CCCAACAGCTATGCTCTTACTCAAATCCATCCGAAGACATCAGGATCGGT478660.15625864582913368No Hit
GGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTT474550.15491693556640493No Hit
CCACGCAAAATGACATTGCAATCCGCCAGCAAGCACCCAAGGCCTTTCCG470860.15371233438161927No Hit
GCGCCCGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC470740.15367316035934983No Hit
CTTTTGTTCTACTGGAGATTTCTGTTCTCCATGAGCCCCCCTTAGGACAT467690.15267748729333458No Hit
CCCTTTTGTTCTACTGGAGATTTCTGTTCTCCATGAGCCCCCCTTAGGAC467610.1526513712784883No Hit
CCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGG465750.15204417393331177No Hit
TGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT463290.151241106476788No Hit
GCGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGAC461610.1506926701650157No Hit
ACCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGGTCTAGGCTT459880.15012791134396442No Hit
GCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCCC454930.14851198292534948No Hit
ACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCC452730.1477937925170762No Hit
CCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATT449400.14670671339909888No Hit
GCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGA442200.14435627206293175No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGC442140.14433668505179703TruSeq Adapter, Index 7 (98% over 50bp)
CCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGT442000.144290982025816No Hit
CTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC437340.14276972416101893No Hit
GCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC436720.14256732504596012No Hit
CGCCGCTTCACTCGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCT426770.13931914569945134No Hit
CAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTAAA425910.1390383985398536No Hit
GCCCAGTGAAATGCGAGATTCCCCTACCCACAAGGAGCAGAGGGCACAAA425080.13876744488582324No Hit
CCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGATA415990.13580001269891223No Hit
CCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGAGCC412890.13478801712361801No Hit
GTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCAC404370.1320066615424869No Hit
ATGATTTATCCCCACGCAAAATGACATTGCAATCCGCCAGCAAGCACCCA402070.13125582611565575No Hit
GCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAG396870.12955828515064616No Hit
GCCGACTTCCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCTT393690.12852017356050566No Hit
GACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTCGTT392140.12801417577285856No Hit
CCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAATG389080.12701523820498753No Hit
CCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAATGA385140.12572902447380718No Hit
CGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCTCCGCTTATTGAT383270.1251185626267749No Hit
CCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGGTCTAGGCTTC380980.12437099170179951No Hit
CGGGTGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACT376740.12298684291494552No Hit
GGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATT369810.12072454312888464No Hit
GCCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAA366460.11963093500719578No Hit
GCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAA366000.11948076792182956No Hit
ATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAG363740.11874299050242153No Hit
GCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCA355300.11598775093613672No Hit
TTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTT354430.11570373927468319No Hit
CCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTATTCTA353230.11531199905198866No Hit
CCGCTTCACTCGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCTCC352500.11507369041651616No Hit
GCGCTTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC351400.11471459521237952No Hit
ACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGAC338820.1106078518777986No Hit
CGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT335810.1096252368192065No Hit
GGCCCAGTGAAATGCGAGATTCCCCTACCCACAAGGAGCAGAGGGCACAA333500.10887113689051955No Hit
CGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGCCATAATCCAGCG329350.10751636862036765No Hit
GGGTGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTT328830.10734661452386669No Hit
TACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTA326940.10672962367312283No Hit
GGCGGCATATAACCATTATGCCAGCATCCTTGACTTACGTCGCAGTCCTC317510.10365119842311503No Hit
CGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGACGGAGTTTC315740.10307338159464059No Hit
GGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTC315560.10301462056123642No Hit
CACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTT311920.10182634188572971No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)