FastQCFastQC Report
Wed 20 Jul 2022
HGCNNBGXM_n01_12268_totalRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCNNBGXM_n01_12268_totalRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23112037
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC6124312.649835667881632TruSeq Adapter, Index 5 (100% over 50bp)
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGC5024692.1740576133553264No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT3136601.357128322354278No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC2735241.183469894929642No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTG2558111.1068301768468094No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACT2433701.0530010833748664No Hit
GTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACT2346451.0152501919238015No Hit
ACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCC2210440.956402068757505No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTT2087130.9030489177565786No Hit
CCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTC1839470.7958926337821283No Hit
ATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAG1779630.770001363358842No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA1723340.7456460890920172No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG1644380.7114820731725204No Hit
CCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAAT1509540.653140179725396No Hit
GGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGC1480630.6406315462371405No Hit
CCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTA1420940.6148051770599018No Hit
GCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTT1351210.5846347511472052No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC1341120.5802690606630648No Hit
CAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCAC1312210.5677604271748093No Hit
CCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCC1302930.5637452034193264No Hit
CGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCT1285850.5563551148693644No Hit
ACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAG1275100.5517038589026142No Hit
CCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTAA1198950.5187556596590772No Hit
GCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCC1163100.5032442618536825No Hit
CGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCGCTGC1112820.48148936417850147No Hit
GCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC982440.42507720111386116No Hit
CACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGC964590.4173539528341877No Hit
CAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCT938580.40610007676952053No Hit
GCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTC932910.4036468096689184No Hit
GCGGCATATAACCATTATGCCAGCATCCTTGACTTACGTCGCAGTCCTCA929770.40228821025165373No Hit
CCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCA922730.39924217843714943No Hit
CTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTC906720.39231505210899414No Hit
CGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTA904840.3915016231585299No Hit
ACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCA902420.39045454972229404No Hit
CGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCCA880640.3810308887961715No Hit
GTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTAC871420.37704162553910764No Hit
GCCGCTTCACTCGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCTC870950.3768382683014916No Hit
CCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAA864250.3739393459780287No Hit
CCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGCCATAATCCAGC838570.3628282526546665No Hit
GCGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCC798450.3454693327117813No Hit
GCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCTCCGCTTATTGATA796890.34479435975288547No Hit
CTCTTACTCAAATCCATCCGAAGACATCAGGATCGGTCGATTGTGCACCT787300.34064500675557075No Hit
CGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAAGGTAT678420.29353535562443067No Hit
CGCCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACG664220.287391370998584No Hit
GTCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCA652100.28214735031793176No Hit
GCGGTAACATTCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA625890.2708069392585344No Hit
CCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCG618950.26780417494139525No Hit
AAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACC608400.2632394539693753No Hit
CTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGATAA592980.25656760587567423No Hit
GCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAG580790.2512932979468664No Hit
GCTACTACCACCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGG579340.2506659192350722No Hit
CCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTT551010.23840823723153434No Hit
TGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAA549800.23788470051341648No Hit
CGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCA543250.23505067943600128No Hit
GTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTA538930.23318152354982816No Hit
GTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCG530870.22969416326219966No Hit
GCGCCATCCATTTTCAGGGCTAGTTCATTCGGCCGGTGAGTTGTTACACA529980.22930908253565013No Hit
CCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAA522170.22592989099143446No Hit
GCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTT517970.22411265610209952No Hit
GCCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGA517860.2240650618549979No Hit
CCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCTT500540.21657113131135952No Hit
TGCCGCTTCACTCGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCT489220.21167325060962822No Hit
CGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTT487800.21105885214704354No Hit
GTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGT485200.2099338972155505No Hit
CCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTA480980.20810800882674255No Hit
CCCAACAGCTATGCTCTTACTCAAATCCATCCGAAGACATCAGGATCGGT472290.2043480633057138No Hit
CGCGTCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT470230.2034567528599924No Hit
GCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGT468960.2029072556434554No Hit
GCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCA468590.20274716590320446No Hit
GCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGG468180.20256976916400748No Hit
CCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGTGGAA460880.1994112418563539No Hit
CGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTA450640.19498065012616586No Hit
TACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGC443860.1920471138048109No Hit
CCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGG441450.19100436711831156No Hit
CTCGTTCGTTATCGCAATTAAGCAGACAAATCACTCCACCAACTAAGAAC439340.19009142292390757No Hit
GCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCA425430.1840729140404197No Hit
CCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGT424360.18360995181861295No Hit
CTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCT420070.18175377618164942No Hit
GTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCT417290.18055093975489914No Hit
CCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCAT415920.17995817504099704No Hit
TGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT413140.17875533861424678No Hit
CGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTC411010.17783374092036977No Hit
CCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAATA401400.17367573442358195No Hit
GCGCCCGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC394410.17065133635776025No Hit
CTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGCTATC389900.16869997222659344No Hit
ATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCA377460.1633174955543728No Hit
GCGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGAC377430.1633045153051633No Hit
CGGGTGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACT377260.1632309605596426No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC374900.1622098476218258TruSeq Adapter, Index 5 (98% over 50bp)
CGCTTCACTCGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCTCCG373090.16142670591951716No Hit
GCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT370790.16043155348011948No Hit
CCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATT370720.1604012662319639No Hit
ACCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGGTCTAGGCTT367080.1588263293278736No Hit
CTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC358160.15496686856290512No Hit
CCCTTTTGTTCTACTGGAGATTTCTGTTCTCCATGAGCCCCCCTTAGGAC344980.14926421241018262No Hit
CCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAATG330790.14312455453407244No Hit
GCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAAGGTATT321430.13907471678069744No Hit
CGCCGCTTCACTCGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCT319800.13836945657364602No Hit
CCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGATA316510.13694595591033365No Hit
GGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATT316480.1369329756611241No Hit
CGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCTCCGCTTATTGAT315380.13645703319010782No Hit
CTTTTGTTCTACTGGAGATTTCTGTTCTCCATGAGCCCCCCTTAGGACAT312910.13538832600518944No Hit
GCCCAGTGAAATGCGAGATTCCCCTACCCACAAGGAGCAGAGGGCACAAA311380.13472633329550313No Hit
CCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGGTCTAGGCTTC306720.13271006791828865No Hit
GCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC302170.13074139678817578No Hit
GCGCTTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC301320.1303736230605723No Hit
ACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGAC300650.130083730828226No Hit
CGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT300450.12999719583349575No Hit
GGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTT300170.12987604684087345No Hit
GCCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAA296660.12835735768335782No Hit
CCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGAGCC296250.12817996094416081No Hit
GTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCAC296060.1280977526991671No Hit
GGGTGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTT288200.12469692740626886No Hit
CCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAATGA286300.12387484495633162No Hit
GACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTCGTT283360.12260278053379717No Hit
CCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTATTCTA278560.12052594066027153No Hit
CAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTAAA276750.1197427989579629No Hit
GCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCCC264930.114628580769406No Hit
GCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGA263480.1140012020576118No Hit
CTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACTA263350.11394495431103714No Hit
GGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTC260040.11251280014825174No Hit
GCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAG257780.1115349547078001No Hit
TGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT256360.11092055624521543No Hit
TACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTA255160.11040134627683401No Hit
CGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGCCATAATCCAGCG252100.10907736085746143No Hit
CGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACAGTTATACCATGT251850.10896919211404862No Hit
TTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTT251790.10894323161562956No Hit
CGTCAGTGTAGCGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACC251310.10873554762827699No Hit
CCGCTTCACTCGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCTCC250000.10816874341279395No Hit
CCCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAG244330.10571547631219179No Hit
CGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGACGGAGTTTC241930.10467705637542896No Hit
GGCCCAGTGAAATGCGAGATTCCCCTACCCACAAGGAGCAGAGGGCACAA240960.10425736165098731No Hit
GGCGGCATATAACCATTATGCCAGCATCCTTGACTTACGTCGCAGTCCTC239050.10343095245131358No Hit
GCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGAT238730.1032924964597452No Hit
CTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTTGAGCT238590.10323192196343403No Hit
CACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTT234690.10154448956619444No Hit
CCGCCGTTTACCCGCGCTTGGTTGAATTTCTTCACTTTGACATTCAGAGC232580.10063154537179046No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)