FastQCFastQC Report
Sun 15 Jan 2017
HGCM3BGX2_n01_hho5_mchx_d4_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCM3BGX2_n01_hho5_mchx_d4_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16758681
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT463750.27672225517032034No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC344700.20568444497511468No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCA301100.17966807769656812No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGGCCGTTCACGGA210940.12586909435175717No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGT184130.10987141529813713No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT172300.10281238720398103No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG167680.10005560700152953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG150150.035.9743585
TACTCTA87800.033.2750054
CTCTAGT95150.031.0304266
CTAGTTG95600.030.3397228
TCTAGTT100500.029.9243857
GCTGACT84500.029.6439571
GTACTCT100100.029.1518963
ACCTCTA103850.027.66521517
CTAGCAG92450.027.3445388
GTTACCT105500.027.1328414
TGTACTC109600.026.7510092
TAGTTGT109500.026.519569
TTGTACT107950.026.4645161
CTGACTC94050.026.4428522
CTAATGC107700.026.33215721
ACTACCC98200.025.71126
GACTCTA101700.025.5050474
TACCTCT117400.024.67685916
CTACCCT102050.024.6370857
TACCCTA103200.024.5629868
Produced by FastQC (version 0.11.2)