FastQCFastQC Report
Sun 15 Jan 2017
HGCM3BGX2_n01_hho5_mchx_d4_7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCM3BGX2_n01_hho5_mchx_d4_7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17944444
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT500150.2787213691324178No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC374580.20874427761595735No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCA322630.17979381250263313No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGGCCGTTCACGGA234370.13060867196553985No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGT205550.114547990453201No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG196050.10925387267501852No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACAT192820.10745387263043649No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT189000.10532508000805152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG170950.037.9717065
TACTCTA104450.034.0126764
GCTGACT89700.033.155561
CTCTAGT110400.032.522876
TCTAGTT115300.031.8857297
GTACTCT111900.031.6559093
CTAGTTG112850.031.2943298
CTGACTC103850.028.7960622
CTAGCAG104650.028.5065168
TTGTACT124350.028.3283651
TAGTTGT127350.028.0828389
GACTCTA109750.027.9388964
GTTACCT124850.027.92704614
TGTACTC127400.027.8590582
ACCTCTA128250.026.8926817
CTAATGC132700.025.7390521
TTACCTC137550.025.49940315
ACTACCC116250.025.3973246
TGTTACC138400.025.26754613
TACCTCT138050.025.08255816
Produced by FastQC (version 0.11.2)