FastQCFastQC Report
Sun 15 Jan 2017
HGCM3BGX2_n01_hho5_mchx_d4_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCM3BGX2_n01_hho5_mchx_d4_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15787106
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT453660.28736109075342875No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACAT323280.2047747066498445No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC316090.20022035704327315No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG310540.19670482987825633No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCA306990.19445615934928162No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGGCCGTTCACGGA218810.1386004502661856No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGT186720.11827373554089014No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT174910.11079294710506155No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATC169680.10748011700181148No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGATGTCCCAGGCGA160220.10148788511333236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG197100.042.1227045
TACTCTA144350.040.407364
CTCTAGT148100.039.6396566
GTACTCT151000.038.6509133
TTGTACT151950.038.3326721
TCTAGTT156450.037.7625927
CTAGTTG157050.037.113238
TGTACTC157750.036.9973072
TAGTTGT169300.034.468589
GTTACCT170250.033.60805514
ACCTCTA173050.032.8860417
GCTGACT77550.032.3926851
TTACCTC178450.032.00584415
TACCTCT182000.031.38165516
TGTTACC183850.031.36552813
CTAATGC179800.031.23822821
CCTCTAA187100.030.50935718
GACACCT181450.029.69041330
GTTGTTA198100.029.49231711
CTAGCAG84700.029.480528
Produced by FastQC (version 0.11.2)