FastQCFastQC Report
Sun 15 Jan 2017
HGCM3BGX2_n01_hho5_mchx_d4_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCM3BGX2_n01_hho5_mchx_d4_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17600892
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT364340.20700087245578233No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC263640.1497878630242149No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCA240740.1367771587939975No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACAT232650.13218080083668488No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG205870.11696566287663149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG167600.037.4558565
TACTCTA114900.036.5337374
CTAGTTG125000.033.2467168
CTCTAGT126050.033.137326
GTACTCT126600.033.0212673
TCTAGTT129400.032.809287
TTGTACT135400.030.580121
TGTACTC138850.030.0589752
ACCTCTA138050.029.78087817
GTTACCT138100.029.76857214
TAGTTGT143000.029.3992969
CTAATGC140350.028.98135821
TACCTCT148000.027.87106116
TTACCTC150400.027.35720415
GACACCT143150.026.95515630
GCTGACT79750.026.8248671
GGACACC146850.026.3937429
CCTCTAA156700.026.30329118
TGTTACC159050.026.216113
TGGACAC149350.026.1139928
Produced by FastQC (version 0.11.2)