FastQCFastQC Report
Sun 15 Jan 2017
HGCM3BGX2_n01_hho5_mchx_d4_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCM3BGX2_n01_hho5_mchx_d4_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16301169
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC362480.22236442061302472No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT359380.22046271650824553No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCA306460.1879987870808529No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG293100.17980305584219144No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACAT276470.16960133349945639No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGGCCGTTCACGGA239820.14711828335746963No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGT191020.11718178003062234No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT170860.10481456882018705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG193000.040.7117085
TACTCTA129050.038.9430354
CTCTAGT132350.037.814856
GTACTCT134800.037.2821163
TTGTACT135700.036.7905961
CTAGTTG134300.036.774438
TCTAGTT139900.035.746477
TGTACTC143700.034.9732742
TAGTTGT149000.033.539749
ACCTCTA147400.033.34450517
GCTGACT88050.032.9545021
GTTACCT149200.032.84784314
CTAATGC153850.031.6634821
TACCTCT158900.030.97383516
ACTACCC156300.030.8508476
CCTACAC160800.030.82101811
CCTCTAA160150.030.53993818
TGTTACC162050.030.43457813
CTAGCAG95850.030.1170398
TTACCTC163650.029.9902315
Produced by FastQC (version 0.11.2)