FastQCFastQC Report
Sun 15 Jan 2017
HGCM3BGX2_n01_hho5_mchx_d4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCM3BGX2_n01_hho5_mchx_d4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17137771
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT463100.2702218392345189No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC370860.21639920384045278No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACAT361550.21096675874593024No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCA326940.19077160034405874No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG315850.18430051375992829No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGGCCGTTCACGGA226620.13223423279491833No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGT203280.11861519213904773No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATC201220.11741316884208572No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT196130.11444312098697082No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCT171400.1000130063588783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG228750.040.9974985
TACTCTA166550.039.9689944
GTACTCT171000.038.828353
CTCTAGT172200.038.797456
TTGTACT172700.038.3188551
TCTAGTT179600.037.4265067
TGTACTC178700.037.348512
CTAGTTG180750.036.730348
TAGTTGT195750.034.179639
ACCTCTA197450.033.0307417
GTTACCT197600.033.02167514
GCTGACT84400.032.581581
TACCTCT207550.031.45550216
CTAATGC205550.031.45387521
TTACCTC209450.031.31825615
TGTTACC211000.031.08810213
CCTCTAA212150.030.71075818
GACACCT207850.029.75680230
GGACACC209300.029.53398929
ATGCTGG216850.029.32889624
Produced by FastQC (version 0.11.2)