FastQCFastQC Report
Sun 15 Jan 2017
HGCM3BGX2_n01_hho5_mchx_d4_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCM3BGX2_n01_hho5_mchx_d4_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15059048
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACAT299550.19891695676911317No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATC156430.10387774844731221No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG151110.10034498860751358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG164650.037.415475
TACTCTA147650.037.2150084
CTCTAGT150300.036.3974846
GTACTCT153850.035.7601243
CTAGTTG153100.035.5475738
TCTAGTT157700.035.0574237
TTGTACT160500.034.3103331
TGTACTC167850.032.880312
TAGTTGT166550.032.8007939
GTTACCT167000.032.25785414
ACCTCTA169800.031.84863717
CTAATGC168500.031.61266521
TACCTCT176550.030.72773216
TTACCTC176750.030.57598715
GACACCT168500.029.9248130
GGACACC169550.029.86152529
CCTCTAA181100.029.84314318
TGTTACC183800.029.4784413
TCTAATG182550.029.28831320
TGGACAC174750.029.05222728
Produced by FastQC (version 0.11.2)