FastQCFastQC Report
Sun 15 Jan 2017
HGCM3BGX2_n01_hho5_mchx_d4_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCM3BGX2_n01_hho5_mchx_d4_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14093607
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACAT361310.25636446368910387No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT335150.2378028562879609No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCA265420.18832652279859938No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC234380.1663023525489252No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG197850.14038279909465334No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATC175440.12448197257096782No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT173440.12306288943632385No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGGCCGTTCACGGA167690.1189830254242225No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGT151140.10724011248504374No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCT145580.10329506137073355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG193250.041.5524145
TACTCTA150450.041.543394
CTCTAGT155600.040.0791556
TTGTACT155150.040.074661
GTACTCT156400.039.918823
TGTACTC161400.038.7894482
TCTAGTT164500.038.200237
CTAGTTG164750.037.451438
TAGTTGT174600.035.5557829
GTTACCT176850.034.63549414
ACCTCTA179500.034.10591517
TTACCTC184350.033.3200615
CTAATGC184000.032.99906521
TACCTCT187650.032.7527116
CCTCTAA189050.032.4016218
TGTTACC191500.032.21996713
GACACCT186700.031.2603730
GGACACC189300.030.83112529
TCTAATG196800.030.79571220
ATGCTGG196850.030.67742224
Produced by FastQC (version 0.11.2)