FastQCFastQC Report
Sun 15 Jan 2017
HGCM3BGX2_n01_attga1_rice_-chx_d1_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCM3BGX2_n01_attga1_rice_-chx_d1_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16120068
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT631200.3915616236854584No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC615640.38190905894441635No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCA399300.24770366973638075No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGT276450.17149431379569863No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGGCCGTTCACGGA261150.16200303869685911No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGATGTCCCAGGCGA259390.1609112318881037No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT243190.15086164648933242No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGAT227380.1410539955538649No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCT226900.14075623006056798No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGG217800.13511109258348042No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC217230.13475749606019033No Hit
GTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCTTGATCTCGCCCTTCAGGGCGCCG203620.12631460363566704No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCCATGTAGGTGGTCTTGACCTCGGCGT195520.12128981093628141No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGG180970.11226379442071832No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCTTGATCTCGCCCTTCAGGGCGCCGTC167670.10401320887728266No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTC165400.10260502623189927No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGAAGCGCATGAACTCCTTGATGACGT162020.10050826088326675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG148800.043.023335
CTGACTC106000.041.8152162
GCTGACT107300.041.7398451
GACTCTA111750.040.86724
TGACTCT114950.038.589613
TACTCTA57850.034.5230834
CTCTAGC131300.034.282156
CTAGCAG140650.031.8524748
GTACTCT63650.031.269133
GTTACCT62500.030.62555914
CTCTAGT65850.030.5899896
TTACCTC66850.028.58122315
CTGTTAC64050.028.4978681
TCTAGTT70800.028.350687
TGTTACC68150.028.1876613
CTAGTTG72400.027.5812388
TAGCAGA165600.027.0741029
CCTCTAA73100.026.56430218
TCTATCG167500.026.37602816
TACCCTA69200.026.364738
Produced by FastQC (version 0.11.2)