FastQCFastQC Report
Sun 15 Jan 2017
HGCM3BGX2_n01_attga1_rice_-chx_d1_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCM3BGX2_n01_attga1_rice_-chx_d1_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14234466
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT551930.3877419778163789No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC426270.29946328861230204No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCA349790.24573454318553295No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGGCCGTTCACGGA241920.1699536884629181No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGT232460.16330784730526596No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACAT215430.15134392818107822No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT211790.14878675462781674No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG206110.1447964398523977No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCT186310.13088653975498624No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGATGTCCCAGGCGA185730.13047907803496106No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGG166930.11727169814448957No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGAT162730.11432111327534168No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCCATGTAGGTGGTCTTGACCTCGGCGT156350.10983903435506467No Hit
GTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCTTGATCTCGCCCTTCAGGGCGCCG155020.10890468247983451No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGG145460.10218858930148837No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC145110.10194270722905939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG158500.042.7399945
TACTCTA94650.040.5238764
GTACTCT98800.038.7869343
CTGACTC76300.038.7423672
CTCTAGT100550.038.384966
TCTAGTT103400.037.256587
GCTGACT81050.036.7805981
GACTCTA82750.036.5559734
TGACTCT84200.035.4349943
CTAGTTG109350.034.8507658
GTTACCT109100.034.17207314
TTACCTC110050.033.84610415
TGTTACC111650.033.57694213
TTGTACT116600.032.7868161
TAGTTGT118750.032.324059
CCTCTAA116250.032.28035718
GTTGTTA117550.032.09636711
TGTACTC121600.031.6563222
ACCTCTA118450.031.56388917
TCTAATG118050.031.32409320
Produced by FastQC (version 0.11.2)