FastQCFastQC Report
Sun 15 Jan 2017
HGCM3BGX2_n01_attga1_rice_-chx_d1_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCM3BGX2_n01_attga1_rice_-chx_d1_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14833135
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT525050.35397102500583993No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCA394830.2661810871403786No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC372430.25107976162827345No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT257030.1732809685882317No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGGCCGTTCACGGA248250.16736178832054047No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGT221710.14946941425396587No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCT183020.12338591942970922No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGATGTCCCAGGCGA181920.12264433648045406No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGG165630.1116621671683026No Hit
GTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCTTGATCTCGCCCTTCAGGGCGCCG159070.1072396361254718No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCCATGTAGGTGGTCTTGACCTCGGCGT158560.10689581130354439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG111400.039.228635
CTGACTC74050.037.7760242
GACTCTA81650.035.780374
GCTGACT82200.034.67031
TGACTCT87200.032.0397453
TACTCTA49500.031.9834334
CTCTAGC92700.030.9191236
CTAGCAG98350.028.8246338
GTTACCT53800.028.7830114
GTACTCT56500.027.9600243
CTCTAGT57950.027.7951686
TTACCTC56050.027.6897715
TGTTACC58300.026.85694113
TCTATCG107900.025.603516
TCTAGTT62850.025.5709557
CTAGTTG62550.025.3076448
TAGCAGA114700.024.7458729
CTATCGA113300.024.44582617
CTAATGC62100.024.27922421
TCTAATG63250.024.16132720
Produced by FastQC (version 0.11.2)