FastQCFastQC Report
Tue 19 Jul 2022
HGCL3BGXM_n01_R20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCL3BGXM_n01_R20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34453058
Sequences flagged as poor quality0
Sequence length130
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT516572914.993528295804687No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA509216214.780000080108998No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG427349712.40382493768768No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC402866911.69321167369236No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC365590510.611264172834819No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA33044489.591160238954696No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT28726668.337912994544636No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG20130885.842987870626753No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)