FastQCFastQC Report
Tue 19 Jul 2022
HGCL3BGXM_n01_R17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCL3BGXM_n01_R17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29481853
Sequences flagged as poor quality0
Sequence length130
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT485715416.475063490751413No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG392721513.320787536658568No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA371291512.593899711798983No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA315320710.695416600849343No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC28662789.722177232211287No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT26346138.936388767693808No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC26119178.859405818216379No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG22243627.544851404014532No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)