FastQCFastQC Report
Tue 19 Jul 2022
HGCL3BGXM_n01_R14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCL3BGXM_n01_R14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29579402
Sequences flagged as poor quality0
Sequence length130
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT429225214.510949207154358No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA424209414.341378503865629No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA354376311.980509274663497No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG340094511.497680040996096No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC334819411.31934310233858No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC305359410.323379762714607No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT23924098.088091165602334No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG18597736.287392152146958No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)