FastQCFastQC Report
Wed 5 Dec 2018
HGCG7BGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCG7BGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20589537
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG648510.3149706571837919No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC467670.22713963893408579No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG450010.218562466946197No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA413850.20100014876487993No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT219360.10653954967515783No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA575700.069.0768051
GTTGTAT327500.069.04117612
AGTAGGT324250.069.031257
TAGGTTG322000.068.743589
TAGTAGG322900.068.693196
GAGGTAG598200.068.605432
TGTATAG400650.068.5309614
GACTGAT518700.068.52444511
GGTAGTA540400.068.501284
TTGTATA402750.068.4514813
TATAGTT358700.068.4475416
CAGACTG517750.068.31319
GTAGTAG538450.068.254965
CTGATGT519400.068.1414213
AGGTAGT544300.067.998433
GTATAGT403100.067.72217615
GGGCTAT60900.067.5659315
GGCATAG106150.067.5412713
AAGATGC106350.067.4835
GCATAGC106000.067.2408914
Produced by FastQC (version 0.11.5)