FastQCFastQC Report
Wed 5 Dec 2018
HGCG7BGX9_n01_AH30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCG7BGX9_n01_AH30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11081435
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC740110.6678828148159512No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG590500.5328732244515263No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA586750.5294891861929435No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAACTGTAGGCACCATCA397720.3589065856542948No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAACTGTAGGCACCATC313560.2829597430296708No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTAACTGTAGGCACCATC287990.2598851141571466No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT274250.2474859979776987No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC258250.2330474347410782No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC206500.18634770677263368No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG195730.1766287488939835No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG175920.1587520027866427No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT165710.14953839462127425No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA147580.1331776976537786No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT145790.1315623833916817No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA133940.12086882249455959No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTTAACTGTAGGCACCAT128120.11561679511723888No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA116490.10512176446462033No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTAACTGTAGGCACCAT114140.10300110048924169No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGGT390650.069.295247
GTAGGTT388750.069.293748
TAGGTTG389000.069.2672049
AGCTTAT587000.069.262382
GTTGTAT399850.069.1907712
GCTTATC588600.069.020623
GTTGACA362350.069.01800518
TGAGGTA723900.069.0181
AGGTTGT457600.068.9468410
TAGTAGG391050.068.93036
TATAGTT434000.068.7259316
GAGGTAG744700.068.7115252
AGATGCT123100.068.6501856
TGTATAG492650.068.6428314
GACTGAT593150.068.61877411
GGTAGTA672800.068.615864
AAGATGC123450.068.595115
CTTATCA592300.068.5776444
TGTTGAC388300.068.57136517
CAGACTG592100.068.562549
Produced by FastQC (version 0.11.5)