FastQCFastQC Report
Wed 5 Dec 2018
HGCG7BGX9_n01_AH29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCG7BGX9_n01_AH29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11609785
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA874190.7529769069797589No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC685730.5906483194994567No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG552640.47601226034762917No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT434100.37390873302132643No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAACTGTAGGCACCATC369410.3181884935853679No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG350220.3016593330539713No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC256880.2212616340440413No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAACTGTAGGCACCATCA213430.18383630704616838No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA174710.1504851295695829No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA174540.15033870136268673No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC160210.13799566486373346No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTAACTGTAGGCACCATC154150.13277592995908194No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTAACTGTAGGCACCAT153780.1324572332734844No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTAACTGTAGGCACCATC140370.12090663177655744No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC138730.11949403025120621No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA138600.11938205574005031No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT131660.11340433952911272No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT441350.069.3837368
AGTAGGT444500.069.308097
GTTGTAT458350.069.253712
AGCTTAT642900.069.252922
TGAGGTA891650.069.198371
TAGGTTG442350.069.195249
GCTTATC643550.069.174933
GTTGACA392750.069.0861418
AGGTTGT526800.069.05575610
CAGCACG242700.068.932594
TAGTAGG444650.068.9236
TATAGTT521250.068.9188916
TGTATAG590400.068.914714
GGTAGTA825100.068.8994144
GAGGTAG915450.068.823452
TGTTGAC419200.068.7860317
GTAGTAG823000.068.760585
CTTATCA647600.068.70994
CAGACTG649100.068.708639
TAGTTTG238450.068.680229
Produced by FastQC (version 0.11.5)