FastQCFastQC Report
Wed 5 Dec 2018
HGCG7BGX9_n01_AH28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCG7BGX9_n01_AH28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10586438
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA774780.7318608959878667No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG624030.5894617245196165No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC521630.4927341944476508No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAACTGTAGGCACCATCA514440.4859424860373243No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTAACTGTAGGCACCATC360280.3403222122492948No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAACTGTAGGCACCATC342090.3231398511945189No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT340490.3216284835371444No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG275360.26010637383414514No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT200090.18900597160253524No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC181280.1712379555805267No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA165220.15606760271963052No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTTAACTGTAGGCACCAT158400.1496253980800719No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC153840.14531800025655467No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTAACTGTAGGCACCAT152750.1442883810399683No Hit
CCCCCACAACCGCGCTTGACTAGCTTGCTGTTTAACTGTAGGCACCATCA151230.14285258176546256No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC141410.1335765627683268No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTAACTGTAGGCACCAT136270.12872129416901132No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT124220.1173388064994099No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC115600.10919631324530499No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA107160.10122384885265469No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTAACTGTAGGCACCATC106230.10034536640180579No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA106100.1002225677796441No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT398450.069.361728
AGTAGGT400900.069.276937
AGCTTAT550000.069.193232
GTTGTAT408650.069.18066412
TAGGTTG399050.069.169739
AGGTTGT466200.069.0545210
GCTTATC550650.069.0348053
TAGTAGG401250.068.963936
GAGGTAG767200.068.889292
GGTAGTA692850.068.870564
TGTATAG501150.068.8600914
TATAGTT447300.068.8567816
TGAGGTA748550.068.8343351
GTTGACA341250.068.8224418
GTAGTAG689100.068.773125
AGTAGTT186800.068.560287
TTGTATA503550.068.50410513
CTTATCA555250.068.4691854
GACTGAT556750.068.4589311
CGTAAAT197200.068.423729
Produced by FastQC (version 0.11.5)