FastQCFastQC Report
Wed 5 Dec 2018
HGCG7BGX9_n01_AH27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCG7BGX9_n01_AH27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10055909
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA777080.7727595784727169No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG662030.6583492352605815No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAACTGTAGGCACCATCA565470.5623260910575065No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC491250.48851874057332856No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTAACTGTAGGCACCATC397060.394852419607218No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAACTGTAGGCACCATC393800.3916105446061614No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT331370.3295276438957433No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG255870.2544474099755676No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC181620.1806102262858584No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTTAACTGTAGGCACCAT177150.17616507866171024No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTAACTGTAGGCACCAT160950.1600551476748646No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT152730.151880849359317No Hit
CCCCCACAACCGCGCTTGACTAGCTTGCTGTTTAACTGTAGGCACCATCA149550.14871852957301027No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC138990.13821724122602938No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTAACTGTAGGCACCAT135820.13506486584156638No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG135770.13501514383234772No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA131200.13047055218976225No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT130730.13000316530310685No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTAACTGTAGGCACCATC116120.11547439420941459No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA113540.11290873853373176No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA113290.11266012848763847No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC112730.11210324198438948No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA109920.10930886506630082No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTGAACTGTAGGCACCATC108300.10769787196761625No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAT580300.069.299692
GTAGGTT392000.069.221668
AGTAGGT394150.069.188277
GCTTATC580050.069.182883
GTTGACA352850.069.17992418
GTTGTAT401450.069.15221412
TGAGGTA726800.069.017081
AGGTTGT458900.069.0053310
TAGTAGG393600.068.9915546
TAGGTTG393750.068.922899
TATAGTT438850.068.91048416
GGTAGTA674650.068.838944
CAGCACG196950.068.812594
TGTATAG491450.068.7856514
CGTAAAT197450.068.784219
GTAGTAG671350.068.77115
CTTATCA583850.068.7146154
TGTTGAC381150.068.7111417
GACTGAT587650.068.7031211
GAGGTAG747850.068.692292
Produced by FastQC (version 0.11.5)