FastQCFastQC Report
Wed 5 Dec 2018
HGCG7BGX9_n01_AH26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCG7BGX9_n01_AH26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10982223
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC693960.6318939253009159No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG525520.4785187844027571No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA438440.39922700531577254No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT263780.24018816591140063No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC257830.23477031926960507No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG256730.2337687005627185No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC201210.18321427273877064No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT198520.18076485971920256No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT165830.15099857287545518No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA156340.14235733512240645No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA155150.14127376579404735No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC138580.12618574581849232No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG129460.11788141617594179No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAT685800.069.336542
GTAGGTT436150.069.330188
AGTAGGT439900.069.2702567
GCTTATC685800.069.228183
TAGGTTG436850.069.2271049
GTTGTAT450950.069.197212
AGGTTGT517900.069.1837110
TGAGGTA805250.069.151511
GTTGACA439900.069.1397418
TAGTAGG438450.069.044726
GAGGTAG826400.069.015732
TGTTGAC465050.068.9998417
TATAGTT493500.068.9912716
CAGCACG202350.068.928474
GGTAGTA740100.068.910074
TGTATAG549500.068.8905514
GTAGTAG737200.068.782435
CAGACTG690300.068.7359549
GTATAGT548850.068.7279815
CTTATCA690950.068.691934
Produced by FastQC (version 0.11.5)