FastQCFastQC Report
Wed 5 Dec 2018
HGCG7BGX9_n01_AH25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCG7BGX9_n01_AH25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10436826
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG546320.5234541612555388No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC475050.45516711689933315No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT451550.43265069284474034No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA442190.42368244905108127No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT252170.241615602291348No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT213740.20479406286930527No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA195050.18688631965312058No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG177930.17048286519292358No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC160760.15403150344750408No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGA153810.1473723908015713No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC151720.1453698662792692No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG138440.13264569132416312No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT136820.1310934952829529No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC135550.12987665023830042No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC124870.11964365411476631No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA104540.10016455194328239No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTTG206450.069.020889
GTAGGTT206250.068.9860158
AGCTTAT248500.068.807462
GTAAGGT108500.068.635742
AGTAGGT208600.068.626267
GCTTATC249200.068.524013
AGGTTGT238800.068.4920110
GGATGGC81700.068.2283255
GTTGTAT208100.068.0700512
AGATGCT58250.068.0029456
GGTAGTA331300.067.9674
TGTATAG249200.067.9074714
TATAGTT216750.067.86883516
TGAGGTA357050.067.859861
GACTGAT253700.067.7801111
GTAGTAG330850.067.604665
GTTGACA138950.067.59939618
TAGTAGG210550.067.55896
GAGGTAG368200.067.509742
CAGCACG85600.067.4090044
Produced by FastQC (version 0.11.5)