FastQCFastQC Report
Wed 5 Dec 2018
HGCG7BGX9_n01_AH24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCG7BGX9_n01_AH24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16236178
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG535100.3295726371070827No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA397300.24470044612716121No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC274140.16884515555323426No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT221410.13636830047071424No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT203180.12514028855805842No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTAT246600.068.8225112
AGTAGGT244200.068.779667
AGCTTAT345750.068.736622
GTAGGTT243550.068.7067268
AGGTTGT280200.068.5740410
GCTTATC346500.068.518373
TGAGGTA428400.068.488311
TAGGTTG244950.068.328549
GACTGAT350400.067.9288611
GTTGACA195400.067.89583618
CTGATGT349900.067.81612413
GAGGTAG446200.067.544662
TAGTAGG248450.067.434526
GGATGGC59800.067.4084555
TATAGTT264500.067.4046216
TGTTGAC207500.067.3211617
GTATAGT303200.067.2837915
GGTAGTA405850.067.275434
CAGACTG352500.067.245819
TTGTATA305200.067.2116413
Produced by FastQC (version 0.11.5)