FastQCFastQC Report
Wed 5 Dec 2018
HGCG7BGX9_n01_AH23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCG7BGX9_n01_AH23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14028250
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG796780.5679824639566589No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA618190.4406750663839039No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT363380.25903444834530326No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC360400.2569101634202413No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG299340.21338370787518046No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT228780.16308520307237181No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC180860.12892556092171156No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA174120.12412097018516209No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT427350.069.3295068
AGTAGGT431150.069.3099447
TAGGTTG428050.069.1916059
GTTGTAT431100.069.0916912
AGGTTGT498100.069.0364110
AGCTTAT492050.069.027462
TGAGGTA754500.068.934111
TAGTAGG430400.068.870236
GCTTATC492250.068.837123
TATAGTT460350.068.7279116
GAGGTAG777350.068.664062
TGTATAG522650.068.63042414
GGTAGTA700600.068.628334
GTTGACA286800.068.4796918
GTAGTAG699550.068.37625
AGATGCT133750.068.3626
AAGATGC133850.068.361025
GACTGAT499400.068.26787611
GTATAGT522550.068.26089515
CAGACTG497150.068.2460259
Produced by FastQC (version 0.11.5)