FastQCFastQC Report
Wed 5 Dec 2018
HGCG7BGX9_n01_AH22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCG7BGX9_n01_AH22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11896746
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG596070.5010361656876594No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC595950.5009352977696591No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA426510.3585097975530452No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT246750.20740965638839393No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG217980.18322657304778972No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG211440.177729271516766No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC181160.15227693354132296No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC158050.1328514536664059No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT138070.11605694531933355No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT127540.1072057855147954No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA127510.10718056853529528No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT467250.069.430738
AGCTTAT796100.069.402372
AGTAGGT470000.069.3285757
GTTGTAT485300.069.2987612
GCTTATC796900.069.283243
AGGTTGT547100.069.2171110
GTTGACA495350.069.20013418
TGAGGTA873750.069.1741
TAGGTTG468700.069.171159
TAGTAGG469400.069.126476
TGTTGAC529000.069.0907617
TATAGTT541000.068.953616
TGTATAG604650.068.94697614
GAGGTAG898100.068.942752
GGTAGTA811500.068.903434
CAGACTG801450.068.8671959
GTATAGT603350.068.83340515
CTTATCA801800.068.829294
CAGCACG232300.068.821564
GACTGAT805200.068.80286411
Produced by FastQC (version 0.11.5)