FastQCFastQC Report
Wed 5 Dec 2018
HGCG7BGX9_n01_AH21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCG7BGX9_n01_AH21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13089224
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG800180.6113273025199966No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA584420.4464894175544708No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC549050.41946718919318676No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT344460.26316304159818793No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG274310.2095693373419234No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT192200.1468383458026236No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC183910.1405048916574428No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA158980.12145868998803902No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC147020.11232140270500375No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC140890.10763816097883265No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAT630400.069.302812
GTAGGTT428550.069.293368
TAGGTTG428850.069.204089
AGTAGGT431900.069.159427
GCTTATC631600.069.125483
GTTGTAT437400.069.0993912
AGGTTGT501450.069.0735110
TGAGGTA779050.068.986741
GTTGACA370700.068.96191418
TATAGTT473200.068.86021416
GGTAGTA719500.068.798084
GAGGTAG799500.068.7655642
CTTATCA634750.068.760924
TAGTAGG432650.068.725146
TGTTGAC395100.068.71905517
AGATGCT145450.068.7117846
GACTGAT638500.068.7049611
TGTATAG538550.068.6668114
AAGATGC145800.068.568755
GTAGTAG718300.068.523295
Produced by FastQC (version 0.11.5)