FastQCFastQC Report
Wed 5 Dec 2018
HGCG7BGX9_n01_AH20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCG7BGX9_n01_AH20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18629971
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC967280.519206390605761No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG645860.34667794168869076No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA574000.3081056862622062No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT322950.1733497062341106No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC319690.17159983770237752No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG311560.16723590176281003No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT273190.14664005649820927No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC242040.1299196869388578No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG234500.12587244499736472No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT208730.11203989528486115No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA193930.10409570685858824No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAT777200.069.2519762
GTAGGTT400050.069.0878
GCTTATC779150.069.05553
AGTAGGT403100.069.01427
AGGTTGT466600.068.9601410
TAGGTTG401200.068.897699
GTTGTAT409350.068.8935612
GTTGACA509400.068.8448118
TGTTGAC540000.068.73579417
TGAGGTA734600.068.628781
CAGACTG785750.068.442599
GACTGAT788900.068.3868411
TAGTAGG404500.068.3779456
CTTATCA787250.068.331664
TGTATAG502600.068.3156814
TATAGTT451400.068.3109116
GAGGTAG757500.068.290322
GGTAGTA683750.068.26564
AGATGCT151300.068.136766
GTATAGT501800.068.0606915
Produced by FastQC (version 0.11.5)