FastQCFastQC Report
Wed 5 Dec 2018
HGCG7BGX9_n01_AH19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCG7BGX9_n01_AH19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16034429
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC775980.48394613864952724No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG399180.2489518023996988No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA340310.21223705565068762No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC268550.1674833572183955No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG266440.16616743882803686No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC210550.13131119293365545No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG202880.12652773603600104No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT200080.12478149362225496No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT196410.12249266874423778No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGGT375500.069.098777
GTAGGTT372050.069.054888
GTTGTAT381750.069.00606512
AGCTTAT475900.069.004162
AGGTTGT437950.068.93088510
TAGGTTG372850.068.831639
GCTTATC477300.068.765133
TGAGGTA654450.068.620851
TAGTAGG375350.068.4644856
TGTATAG455250.068.3797414
GTTGACA305750.068.2985518
TATAGTT407150.068.2052216
GACTGAT481350.068.19829611
GAGGTAG674000.068.187852
AGATGCT111400.068.162216
GTATAGT454550.068.136715
TGTTGAC325950.067.9674917
TTGTATA458750.067.89596613
GGTAGTA605400.067.820444
CAGACTG483050.067.791039
Produced by FastQC (version 0.11.5)