FastQCFastQC Report
Wed 5 Dec 2018
HGCG7BGX9_n01_AH18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCG7BGX9_n01_AH18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11637184
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG395440.3398072935858022No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC378140.3249411541486325No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA334900.2877843986998917No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT188210.16173156667454944No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG177830.15281188301224763No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC133810.11498486231720664No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAT513650.069.1925662
GCTTATC514550.069.0345463
GTAGGTT259600.069.0146568
AGGTTGT301600.068.86034410
AGTAGGT261900.068.847417
GTTGTAT266750.068.8185412
TAGGTTG260250.068.8153849
TGAGGTA479950.068.729941
GACTGAT517600.068.6004411
TGTTGAC344100.068.5902117
CAGACTG517400.068.578449
GTTGACA325200.068.5497718
TATAGTT298050.068.5366716
TAGTAGG261700.068.525946
GAGGTAG495700.068.330662
CTTATCA520200.068.304934
CTGATGT519250.068.2239313
GATGTTG518450.068.1989115
GTATAGT330850.068.07715
GGTAGTA451150.068.071854
Produced by FastQC (version 0.11.5)