FastQCFastQC Report
Wed 5 Dec 2018
HGCG7BGX9_n01_AH17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCG7BGX9_n01_AH17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13544786
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC664920.4909047658634104No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG388080.2865161546295379No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA312220.23050936352925766No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC230050.1698439532377994No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG216970.1601871007781149No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT174840.12908288104367246No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC168990.12476387592982273No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG153460.11329820936262854No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAT722950.069.393112
GCTTATC724700.069.192233
GTTGTAT312400.069.056712
GTTGACA467700.069.0178418
AGTAGGT310050.069.0034947
GTAGGTT308650.068.9768758
TGAGGTA586800.068.845171
TGTTGAC493400.068.8222717
GACTGAT730400.068.8179311
AGGTTGT355350.068.77466610
TAGGTTG309550.068.742429
CTTATCA729200.068.7364354
CAGACTG729750.068.724779
TAGTAGG310400.068.6441046
CTGATGT729900.068.6384713
GAGGTAG603200.068.636752
GATGTTG729400.068.526715
AGATGCT112050.068.517856
TAGCTTA730750.068.49471
ACTGATG731950.068.4661212
Produced by FastQC (version 0.11.5)