FastQCFastQC Report
Wed 5 Dec 2018
HGCG7BGX9_n01_AH16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCG7BGX9_n01_AH16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10396112
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC484210.46576066129337584No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG407370.39184841409942484No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA367120.3531320170463727No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT208030.2001036541353152No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG205320.19749691038342027No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC161320.15517339559250612No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG148680.14301500407075263No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT137350.13211669901209222No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC128920.12400789833737845No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC104450.10047025272524959No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAT456250.069.170742
GTAGGTT275950.069.137798
GTTGTAT282350.069.096412
AGTAGGT277800.069.092067
GCTTATC456450.069.0334553
AGGTTGT323000.068.91585510
TGAGGTA499700.068.836811
GTTGACA296450.068.7522718
GGGCTAT58050.068.7205115
TGTATAG342100.068.6777714
TAGGTTG277900.068.6652459
GACTGAT460200.068.6434911
CAGACTG458800.068.6068959
TATAGTT306850.068.5840516
TAGTAGG278200.068.577966
GAGGTAG513600.068.560562
GGTAGTA463100.068.427514
TAGCTTA459150.068.382811
CTTATCA460750.068.366414
AGATGCT91900.068.2351766
Produced by FastQC (version 0.11.5)