FastQCFastQC Report
Wed 5 Dec 2018
HGCG7BGX9_n01_AH15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCG7BGX9_n01_AH15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10588868
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC502000.4740827820310915No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG469390.4432862889593108No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA395740.3737321118744704No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT222510.2101357765532633No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG214270.2023540193342669No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC165940.15671174671362417No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT153300.1447746822417656No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA133690.12625523332616856No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC132750.1253675085948753No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC124420.11750075645479763No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT122400.1155930926705291No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA108940.10288163002881894No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT298550.069.143188
AGCTTAT470800.069.1262052
AGGTTGT347450.069.1004810
GCTTATC471000.069.0244453
GTTGTAT302500.068.9228412
TGAGGTA540500.068.9221651
AGTAGGT301400.068.9057167
GTTGACA290900.068.88560518
TAGGTTG299800.068.831559
TGTTGAC307950.068.7588817
TATAGTT331750.068.66566516
GGTAGTA501950.068.637394
GAGGTAG556400.068.63622
TAGTAGG301550.068.5461966
GACTGAT475700.068.53857411
TGTATAG370250.068.47327414
GTATAGT369000.068.4384115
GTAGTAG500500.068.3894355
CTTATCA476100.068.314454
CAGACTG476100.068.303959
Produced by FastQC (version 0.11.5)