FastQCFastQC Report
Wed 5 Dec 2018
HGCG7BGX9_n01_AH14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCG7BGX9_n01_AH14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12026183
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC543040.45154809302336407No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG481890.4007007044546054No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA423740.3523478729701685No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT247920.2061501974483508No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG216490.18001555439493977No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT204400.16996248934512306No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC178580.1484926680393937No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA175290.14575697043692085No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC161810.13454809393803505No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC142170.11821706022600853No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT133440.1109578991106322No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT247550.068.997988
AGTAGGT249400.068.975547
TAGGTTG247950.068.830219
AGCTTAT327550.068.8146062
AGGTTGT287350.068.8053710
GCTTATC327800.068.681973
GTTGTAT246950.068.47098512
TGAGGTA441500.068.35821
GTTGACA194600.068.174418
TAGTAGG250800.068.172496
GAGGTAG454250.068.0946662
TATAGTT268550.068.03007516
TGTATAG298950.067.9376214
GGTAGTA410900.067.897894
TGTTGAC207550.067.87910517
AGATGCT88250.067.765816
GACTGAT334100.067.7562511
CAGACTG333500.067.658089
GTATAGT299050.067.6326615
TAGCTTA331650.067.569231
Produced by FastQC (version 0.11.5)