FastQCFastQC Report
Wed 5 Dec 2018
HGCG7BGX9_n01_AH13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCG7BGX9_n01_AH13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12178658
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC532360.43712533844040946No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG457630.3757638977956356No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA379120.3112986668974529No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT220060.18069314369448589No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG187090.15362119537308627No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC159230.13074511165351715No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT139750.11474991743753704No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC126040.10349251945493503No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAT382650.069.030672
AGTAGGT243000.068.90497
GTAGGTT242050.068.857868
GCTTATC383500.068.8067253
GTTGTAT244400.068.7856212
GTTGACA231450.068.73965518
AGGTTGT281550.068.6570410
TGAGGTA433450.068.637871
TAGGTTG243100.068.589239
GACTGAT387150.068.41559611
TGTTGAC245200.068.318317
TATAGTT268150.068.2382316
GAGGTAG446500.068.203942
TAGTAGG244700.068.12596
CAGACTG387650.068.089849
TGTATAG302050.067.9948114
TAGCTTA387150.067.958291
GGTAGTA405450.067.929734
AGATGCT88000.067.877426
CTGATGT388500.067.8347313
Produced by FastQC (version 0.11.5)