FastQCFastQC Report
Wed 5 Dec 2018
HGCG7BGX9_n01_AH12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCG7BGX9_n01_AH12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13204182
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC578150.4378537042279484No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG361710.2739359393864762No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA248460.18816765779205408No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG184180.13948611129413394No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC165840.12559657235866636No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT142060.10758712656338727No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC140020.10604216149095794No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAT601550.069.177552
GTAGGTT310050.069.10778
GTTGTAT312400.069.0916512
GCTTATC602200.069.0844653
AGTAGGT312900.069.069937
AGGTTGT354550.068.9514410
TAGGTTG310800.068.8846369
GTTGACA377650.068.8053518
TGAGGTA572400.068.771211
TAGTAGG312450.068.699546
GACTGAT607850.068.6121211
CTGATGT607100.068.545213
CAGACTG607400.068.530449
TGTTGAC399550.068.3744417
GAGGTAG589950.068.354792
CTTATCA608450.068.340324
GGTAGTA538750.068.328874
TGTATAG389300.068.3235414
TAGCTTA606600.068.292971
AGATGCT112950.068.15966
Produced by FastQC (version 0.11.5)