FastQCFastQC Report
Wed 5 Dec 2018
HGCG7BGX9_n01_AH11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCG7BGX9_n01_AH11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12875692
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC602750.46813017894494524No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG480780.3734012898102875No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA361960.28111887112552864No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT205210.159377841594844No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG195400.15175883362230164No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC180700.1403419715227733No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG175870.13659071683292828No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC147260.11437055188956058No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAT681350.069.285632
GCTTATC681800.069.182663
GTAGGTT294600.069.142678
AGTAGGT297000.069.0892267
GTTGTAT305350.069.0694812
TGAGGTA560950.068.897831
AGGTTGT344000.068.8365310
GTTGACA421850.068.791518
TGTTGAC447200.068.7637217
GACTGAT688500.068.6902411
CTTATCA687150.068.65934
TAGGTTG296800.068.630159
CAGACTG687350.068.626719
TAGTAGG296850.068.618596
AGATGCT116400.068.603136
GAGGTAG578450.068.4949952
CTGATGT689200.068.4761813
TATAGTT344650.068.4598416
GATGTTG687350.068.4300615
AAGATGC116650.068.423445
Produced by FastQC (version 0.11.5)