FastQCFastQC Report
Wed 5 Dec 2018
HGCG7BGX9_n01_AH10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCG7BGX9_n01_AH10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12471594
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC850860.6822383730580068No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG552670.4431430336811798No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA492530.3949214510991939No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT286700.22988240316354108No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC282670.22665106000083068No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG278690.2234598079443574No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT257970.20684605351970245No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC222610.17849362318882414No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA209370.1678774982572396No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT201510.16157517635676724No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC194230.15573791128864523No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA162460.13026402238559082No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA161130.1291975989596839No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG134660.10797336731776226No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC133430.10698712610432957No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG126580.10149464454984664No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT324000.069.175768
AGTAGGT327350.069.0335547
AGGTTGT378150.068.93085510
TAGGTTG324850.068.908859
GTTGTAT328050.068.8556312
TAGTAGG326850.068.679636
TGAGGTA576400.068.440881
TATAGTT356600.068.37508416
GGTAGTA531700.068.26644
TGTTGAC275350.068.2574217
TGTATAG397500.068.2512614
GAGGTAG593050.068.161812
GTTGACA257450.068.0761118
AGATGCT109700.068.0718466
GTAGTAG530000.068.069715
GACTGAT418450.068.004411
GTATAGT397600.067.9956515
CAGCACG134900.067.986764
CTTATCA415550.067.836824
CAGACTG417200.067.687869
Produced by FastQC (version 0.11.5)