FastQCFastQC Report
Wed 5 Dec 2018
HGCG7BGX9_n01_AH09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCG7BGX9_n01_AH09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12514648
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1185020.9469063772309056No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG765250.6114834392465534No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA673130.5378736980856353No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC389570.31129121650085567No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT382710.3058096400314256No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG368010.29406340473978976No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT316670.25303947821784517No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC307380.24561617713898146No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT273370.21844002324316272No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA264060.21100074089179335No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC241710.19314166886675518No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA228190.18233832865295133No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA220650.17631338891832996No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC185560.14827424630720734No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG163480.13063092146099514No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG159700.12761046095743164No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG149600.11953991834209No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAT732150.069.230422
GTAGGTT450150.069.1721958
AGTAGGT454500.069.161847
TAGGTTG450350.069.110399
AGGTTGT529300.068.9499110
GTTGTAT461800.068.9427212
TAGTAGG451850.068.9404456
GTTGACA464050.068.9120118
GCTTATC735750.068.869983
TGAGGTA819500.068.780221
TATAGTT501800.068.7703516
TGTATAG561300.068.74586514
GAGGTAG841400.068.696912
GGTAGTA755850.068.6958854
GTAGTAG752400.068.58335
TGTTGAC496700.068.5758117
CAGCACG211150.068.5411154
AAGATGC159600.068.5361255
CTTATCA739950.068.464894
CAGACTG741350.068.383549
Produced by FastQC (version 0.11.5)