FastQCFastQC Report
Wed 5 Dec 2018
HGCG7BGX9_n01_AH08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCG7BGX9_n01_AH08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14512528
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC732950.5050463985323577No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG409070.28187370250035004No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA347110.23917955576037475No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC205590.14166380936526013No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT198460.13675081281496923No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG185490.127813706888283No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG182720.12590501117379413No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC168020.11577583175033322No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAT594800.069.2561952
GCTTATC595950.069.116593
GTTGTAT274500.069.04394512
GTAGGTT269050.068.973288
TGAGGTA498250.068.78431
AGTAGGT272150.068.740077
GTTGACA382050.068.6740318
AGGTTGT316200.068.6696310
TGTTGAC405550.068.66759517
GACTGAT601400.068.6431911
TAGGTTG270300.068.6411369
CAGACTG601600.068.510089
CTTATCA601850.068.415784
GAGGTAG514600.068.413912
CTGATGT603350.068.334113
TAGTAGG272050.068.327996
GATGTTG601650.068.30569515
TAGCTTA600650.068.253741
TGTATAG339050.068.2015214
GGTAGTA465950.068.040534
Produced by FastQC (version 0.11.5)