FastQCFastQC Report
Wed 5 Dec 2018
HGCG7BGX9_n01_AH07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCG7BGX9_n01_AH07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11585765
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC616740.5323256599801567No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG397840.3433869062595349No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA308420.26620598639796333No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC187240.16161211624782654No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG174190.15034829378983605No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT172330.14874287541651326No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG155730.1344149479986863No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC149280.12884777138151862No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT121750.10508585320002607No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAT560350.069.135242
GCTTATC561100.068.967673
GTTGTAT286350.068.8748412
GTAGGTT279650.068.873538
AGGTTGT326950.068.7994710
TGAGGTA506100.068.7663651
TAGGTTG280200.068.7383359
TGTTGAC381150.068.7205217
GTTGACA359150.068.6851418
AGTAGGT283000.068.6756067
GACTGAT564800.068.5689811
CAGACTG564900.068.506699
GAGGTAG521450.068.480822
CTGATGT564200.068.4419513
GATGTTG563600.068.4089615
AGATGCT108250.068.402326
GGTAGTA469350.068.373824
CTTATCA566700.068.366424
TAGTAGG282300.068.325276
TATAGTT309750.068.2236516
Produced by FastQC (version 0.11.5)