FastQCFastQC Report
Wed 5 Dec 2018
HGCG7BGX9_n01_AH06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCG7BGX9_n01_AH06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19031790
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC898250.47197347175436466No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG634520.33340006378800946No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA483320.25395404215788425No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG283520.14897179928950458No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC269790.14175755407137217No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT267700.14065939147079703No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC229360.12051415027172956No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG222530.11692541794544813No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAT856650.069.239832
GCTTATC857650.069.117113
GTAGGTT368850.068.866078
AGTAGGT371750.068.844767
TGAGGTA677500.068.83081
GTTGACA553750.068.76556418
TGTTGAC584200.068.7494517
GTTGTAT376750.068.6578812
AGGTTGT429750.068.6014810
TAGGTTG371200.068.505519
CAGACTG865550.068.441179
AGATGCT144600.068.435686
GACTGAT868500.068.43101511
CTTATCA867000.068.371734
TAGCTTA866750.068.206531
TAGTAGG372850.068.201276
GAGGTAG702500.068.159232
CTGATGT869450.068.1369413
GGTAGTA636850.068.080984
GATGTTG869500.068.0024615
Produced by FastQC (version 0.11.5)