FastQCFastQC Report
Wed 5 Dec 2018
HGCG7BGX9_n01_AH05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCG7BGX9_n01_AH05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11256802
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC628460.5582935544215845No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG429160.38124504632843326No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA357650.3177190111365555No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT200570.17817671484316772No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC199020.17679976959708452No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG184840.1642029414748523No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC160570.14264264397650417No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG157200.13964889850598777No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT130930.11631189746430647No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT125780.11173688584022355No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA113730.10103224699164115No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT285650.069.30058
GTTGTAT291900.069.2321912
AGCTTAT483700.069.1874852
AGTAGGT287600.069.181027
AGGTTGT333550.069.0737910
TAGGTTG286600.069.034479
TGAGGTA517400.069.012551
GCTTATC485500.068.937433
TAGTAGG287800.068.768496
GTTGACA304900.068.6821118
CAGCACG134850.068.633474
TGTTGAC322650.068.6299117
GGTAGTA480550.068.606584
GAGGTAG534250.068.542942
GTATAGT354900.068.53557615
TGTATAG356550.068.53490414
GACTGAT489900.068.501111
CTTATCA488550.068.478714
TATAGTT322450.068.4762316
GTAGTAG478300.068.402585
Produced by FastQC (version 0.11.5)