FastQCFastQC Report
Wed 5 Dec 2018
HGCG7BGX9_n01_AH04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCG7BGX9_n01_AH04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16609468
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC693920.41778580746836685No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG459450.27661933542964773No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA398640.24000768718179297No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG242510.14600708463389675No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT241560.14543512170287454No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC228560.13760826054151765No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC194090.11685503713905827No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT184100.1108403953696771No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT457450.069.159868
AGGTTGT532750.069.09270510
AGTAGGT462200.069.0531167
AGCTTAT473900.069.043972
TAGGTTG457850.069.0239
GTTGTAT460800.068.9833912
TGAGGTA786400.068.8978351
TAGTAGG461150.068.649656
TATAGTT488600.068.62173516
GAGGTAG809150.068.592242
GCTTATC476800.068.588473
TGTATAG546900.068.4101414
GGTAGTA727700.068.3816154
GTATAGT544400.068.33678415
TTGTATA548750.068.2241513
GTAGTAG726500.068.095015
TGTTGAC311250.068.0614517
GTTGACA295450.068.0356418
GACTGAT480850.067.9920211
AGGTAGT731350.067.959013
Produced by FastQC (version 0.11.5)