FastQCFastQC Report
Wed 5 Dec 2018
HGCG7BGX9_n01_AH03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCG7BGX9_n01_AH03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13975363
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1130570.8089736202200973No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG551770.39481622051606097No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA462520.3309538364048218No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC338340.24209746823749767No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC284750.2037514159739536No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG264520.1892759422420727No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT257490.1842456614543751No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG245500.17566627786340863No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT217320.15550222201741737No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT199200.14253654806676577No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA185450.13269780541657486No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA160610.11492366960342998No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA150910.10798288387929529No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAT530250.069.0818562
GTAGGTT327550.068.995068
GCTTATC531500.068.866883
GTTGTAT334150.068.84589412
AGTAGGT330950.068.79337
AGGTTGT381350.068.7857910
GTTGACA338050.068.77106518
TAGGTTG328850.068.74369
TGAGGTA586750.068.6643451
TGTTGAC357200.068.5965117
GACTGAT538050.068.4334811
GAGGTAG607550.068.350982
TAGTAGG330500.068.325796
GGTAGTA547350.068.2727664
TATAGTT369800.068.235316
CTTATCA536200.068.2306064
CAGACTG537250.068.229979
TGTATAG409050.068.1214514
AGATGCT129000.067.9807366
GTATAGT408750.067.9312515
Produced by FastQC (version 0.11.5)