FastQCFastQC Report
Wed 5 Dec 2018
HGCG7BGX9_n01_AH02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCG7BGX9_n01_AH02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11954482
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1148250.9605184064018835No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG702530.5876707999560332No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA593770.49669237027585134No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC357830.2993270641086749No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT335400.2805642268732346No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG314220.26284702256442394No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC289520.2421853159342245No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG259200.2168224436658987No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT218870.18308614292112366No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT208770.17463742887395706No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA194850.16299326060301064No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA179210.149910301425022No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA162430.13587372501794726No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC146020.12214665595715482No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG140770.11775499766531079No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAT995150.069.4237062
GCTTATC996100.069.2984543
GTAGGTT460200.069.271348
AGTAGGT463750.069.245177
GTTGACA626800.069.17853518
GTTGTAT470600.069.147312
TGTTGAC667250.069.0208417
TGAGGTA850150.069.0142441
AGGTTGT537200.068.9835210
CTTATCA1000450.068.965664
CAGACTG1001750.068.95919
TAGGTTG462000.068.9569
GACTGAT1004800.068.9259311
TATAGTT526500.068.8891616
TGTATAG586800.068.8715214
GAGGTAG873050.068.81492
CAGCACG225450.068.803874
TAGCTTA999350.068.7812041
GGTAGTA791650.068.7590944
AGATGCT175600.068.749436
Produced by FastQC (version 0.11.5)