FastQCFastQC Report
Wed 5 Dec 2018
HGCG7BGX9_n01_AH01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCG7BGX9_n01_AH01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11660050
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC682450.5852890853812805No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG467990.40136191525765325No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA397310.3407446794825065No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT227740.1953164866359921No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG217100.18619131135801306No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC213740.18330967705970386No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG178960.153481331555182No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC171450.1470405358467588No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT161270.13830987002628634No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT125910.10798409955360398No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA123930.10628599362781463No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA118860.10193781330268739No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAT494200.069.2478562
GTAGGTT292800.069.233968
GCTTATC494700.069.1495363
AGTAGGT295300.069.037117
TAGGTTG293550.068.997189
TGAGGTA531950.068.975981
GTTGACA320000.068.9199518
GTTGTAT300600.068.9033712
AGGTTGT343950.068.87704510
TGTTGAC338050.068.8559217
GAGGTAG547400.068.738362
GGTAGTA492750.068.663334
AAGATGC108800.068.569075
TAGTAGG295600.068.564856
GACTGAT502050.068.50835411
TATAGTT330800.068.4815216
AGATGCT108900.068.475156
CAGACTG500800.068.4703149
CTTATCA500500.068.4390954
TGTATAG368100.068.34080514
Produced by FastQC (version 0.11.5)