FastQCFastQC Report
Wed 5 Dec 2018
HGCFKBGX9_n01_CrossBpool.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCFKBGX9_n01_CrossBpool.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35823617
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG163750.015.83876646
GTATGCC170250.015.37781745
CGTATGC179200.014.49243244
ATGCCGT178350.013.93327747
TCGTATG200000.012.72269543
CTCGTAT219000.011.65092942
TGCCGTC206350.011.51706648
GCCGTCT212500.010.80488849
CTGCTTG182750.010.284183557
TGCTTGA206250.010.011744558
CCGTCTT258500.09.54550950
TAGCATC332350.09.33035736
TCTCGTA273300.09.25925141
GTAGCAT377800.08.89340935
GCTTGAA275700.08.27682559
ATCTCGT328150.08.06352140
GGTAGCA435700.08.02480534
GCATCTC352400.07.86598538
AGCATCT381500.07.42170837
TCTGCTT275450.07.42029556
Produced by FastQC (version 0.11.5)